VIDEO ANSWER: Hello. In this question it has been asked, competent cells are rendered permissible by exposing them to calcium chloride solution. Why do you think the competent cells must be kept on ice during transformation? Okay that's the question.
Competent cells are rendered permeable by a solution: calcium chloride transformation. Competent cells must be kept on ice during cooling.
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Bacterial Transformation Protocol with Competent Cells
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SOLVED: Competent cells are rendered permeable by a solution: calcium chloride transformation. Competent cells must be kept on ice during cooling.
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Bacterial Transformation Protocol with Competent Cells
PDF] An improved calcium chloride method preparation and transformation of competent cells
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